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- Mouse Anti-Rabbit Light Chain-AP
Product short description
Price:
442 EUR
Size:
1 mililiter
Catalog no.:
GEN675060
Product detailed description
Purification method
N/A
Other names
N/A
Gene name
N/A
Other gene names
N/A
Concentration
N/A
Gene name synonims
N/A
Clone
SB62a
Clonality
Monoclonal
Immunoglobulin isotype
IgG2b lambda
Tested applications:
ELISA, Western Blot
Category
Secondary Antibodies
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Also known as
Mouse Anti-Rabbit Light Chain
Latin name
Mus musculus,Oryctolagus cuniculus
Form/Appearance
Alkaline Phosphatase (AP) Conjugate
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term the antibody should be stored at -20 degrees Celsius..
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About
Rabbits are used for polyclonal antibody production by MBS Monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. MBS Monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Specificity and cross-reactivity
Rabbit Light Chain Mouse Anti-Rabbit Light Chain antibody (clone: SB62a) reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50kD). By using our Mouse Anti-Rabbit Light Chain antibody, detection of antigens with molecular weights near 50kD is not obscured by large amounts of reduced and denatured Rabbit IgG heavy chains on the blots. If the antigen of interest has a reduced and denatured molecular weight near 25kD, Mouse Anti-Rabbit IgG Clone: 2A9) may be used to detect the heavy chain of the Rabbit IgG primary antibody without binding to the 25kD band of reduced and denatured IgG Light Chains on Western Blots. This antibody is not recommended for sensitive or quantitative detection of the reduced and denatured Rabbit Light Chains on Western Blots.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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