ACT, Actin (monoclonal)

502 EUR

100 µg

AS16 3141

Mouse

Q96293

Inquire

At1g49240

Monoclonal

45 | 45 kDa

1-2 ug/ml (WB)

Affinity purified

IgG2b, clone mAbGPa (10-B3)

Immunohistochemistry (IHC), Western blot (WB)

Arabidopsis thaliana, Dictyostelium discoideum

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See included datasheet or contact our support service

no confirmed exceptions from predicted reactivity are currently known

Full length recombinant Arabidopsis thaliana Actin-8 (ACT8) expressed in E.coli, Uniprot: Q96293

This antibody is a total IgG fraction purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin

Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

AS13 2640 | anti-ACT | actin, rabbit antibody AS10 681 | Anti-tubulin beta chain, rabbit antibodyAS10 680 | Anti-tubulin alpha chain, rabbit antibodyAS10 702 | Actin-11, mouse monoclonal antibodyAS16 3140 | ACT2,8,11 | Actin 1, 8, 11, mouse monoclonal antibodyAS16 3139 | ACT1,3,4,12 | Actin 1, 3, 4, 12, mouse monoclonal antibodyPlant and algal protein extraction bufferSecondary antibodies

Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.

Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pratense, Ziziphus jujuba