5-hmC, 5-hydroxymethylcytosine (monoclonal)

622 EUR

100 µg

AS16 3164

NA

Mouse

IgG1k

human

Inquire

Monoclonal

see datasheet

Refer to NCBI

Affinity purified

mouse, broad species range

To be added when available

collection of antibodies to epigenetics

2 µg/ml (Dot), 1: 500 (ELISA), 2.5 µg/IP (hMeDIP)

See included datasheet or contact our support service

BSA-conjugated molecule: 5-hydroxymethylcytosine (5-hmC)

To be added when available, antibody released in February 2016.

No confirmed exceptions from predicted reactivity known at the moment

Dot blot (Dot), ELISA (ELISA), hMeDIP (hydroxymethylated DNA immunoprecipitation)

Store at -20°C; for long term storage -80°C is recommened; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Close structural similarity between 5-mC and 5-hmC makes them very difficult to expreimentally distinuish. Therefore affinity-based technology was employed to purify 5-hmC specific antibodies. This antibody is affinity purified and provided in PBS pH 7.4 with 0.05 % sodium azide.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

5-hmC | 5-hydroxymethylcytosine is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. It may have an important roles distinct from that of 5-methylcytosine (5-mC). 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells.