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- MBS Monoclonals
- Flag-Tag monoclonal antibody
Product short description
Price:
243 EUR
Size:
50ug
Catalog no.:
GEN850209
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Purification method
N/A
Conjugation
Flag
Clone
G191
Immunoglobulin isotype
IgG2b
Also known as
Flag-Tag
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Concentration
0.05mg/0.05ml
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Tested applications:
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Form/Appearance
Mouse Monoclonal (IgG2b) 1mg/ml in PBS (pH 7.2) with 50% glycerol. Frozen liquid.
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Storage and shipping
Stable for one year the antibody should be stored at -20 degrees Celsius. from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at Store the antibody at +4 degrees Celsius. for 3 months.
Specificity and cross-reactivity
Recognizes over-expressed proteins containing Flag epitope tag (D-tag) fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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