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- MBS Monoclonals
- FLAG (DYKDDDDK) Tag
Product short description
Price:
332 EUR
Size:
100ug
Catalog no.:
GEN395220
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Conjugation
Flag
Clone
FG4R
Immunoglobulin isotype
IgG2b
Clonality
Monoclonal
Category
Antibodies
Also known as
FLAG (DYKDDDDK) Tag
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Purification method
Protein A/G Chromatography
Form/Appearance
10mM PBS, pH 7.2, with 0.05% Sodium Azide
Tested applications:
Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS)
Storage and shipping
Product should be stored the antibody should be stored at -20 degrees Celsius.. Aliquot to avoid freeze/thaw cycles
Species reactivity
FLAG tagged fusion proteins; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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