Product short description
Price:
663 EUR
Size:
500ug
Catalog no.:
GEN602429
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Also known as
Bu-1
Clone
5K98
Immunoglobulin isotype
IgG1,k
Source organism
Chicken
Purification method
Purified
Clonality
Monoclonal
Category
Antibodies
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Form/Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
Tested applications:
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes chicken Bu-1 (chB6), Mr 70-75kD. Does not crossreact with turkey cells when analyzed by immunofluorescent staining and flow cytometry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.