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- MBS Monoclonals
- Bu-1 (Biotin)
Product short description
Price:
625 EUR
Size:
500ug
Catalog no.:
GEN603027
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Also known as
Bu-1
Clone
5K98
Immunoglobulin isotype
IgG1,k
Source organism
Chicken
Purification method
Purified
Clonality
Monoclonal
Category
Antibodies
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Form/Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Tested applications:
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Specificity and cross-reactivity
Recognizes chicken Bu-1 (chB6), Mr 70-75kD. Does not crossreact with turkey cells when analyzed by immunofluorescent staining and flow cytometry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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