HESCA-1 (Human Embryonic Stem Cell Antigen-1)

691 EUR

100ug

GEN604449

N/A

N/A

N/A

N/A

N/A

IgM,k

9H242

HESCA-1

Purified.

Antibodies

cell, stem

Monoclonal

Mnoclonal antibodies

Human (Homo sapiens)

Mouse (Mus musculus)

Supplied as a liquid in PBS, 0.25 sodium chloride, pH 7.6, 0.1% sodium azide.

Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recognizes H1, BG-01, BG01v, and BF02 human embryonic stem (hES) cell lines. Unreactive for differentiated stem cells in teratomas and common feeder lines such as MEF, D-551, and Hs27. Reactivity with other hES lines has not yet determined.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.