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- MBS Monoclonals
- F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (FITC)
Product short description
Price:
801 EUR
Size:
100ug
Catalog no.:
GEN605325
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Clone
BM8
Other names
N/A
Tissue
cell
Immunoglobulin isotype
IgG2a
Category
Antibodies
Clonality
Monoclonal
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Also known as
F4/80, Pan Macrophages
Host organism
Rat (Rattus norvegicus)
Purification method
Affinity Purified by Protein G affinity chromatography.
Tested applications:
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Form/Appearance
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
Specificity and cross-reactivity
Recognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. F0001-51B is a macrophage marker capable of distinguishing non-destructive from destructive inflammation processes in the pancreas. It is also a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model. Species Crossreactivity: human (heart macrophages). Does not crossreact with mouse granulocytes, mast cells, platelets, lymphocytes, fibroblasts, or endothelial cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Description
For cells, cell lines and tissues in culture till half confluency.Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.Precursor cell, also called a blast cell or simply blast, is a type of partially differentiated, usually unipotent cell that has lost most or all of the stem cell multipotency.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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