CD3, epsilon (Early T Cell Marker) (FITC)

542 EUR

500ug

GEN609205

5K3

N/A

cell

IgG,k

Hamster

Purified

Antibodies

Monoclonal

CD3, epsilon

Anti-FITC Antibody

cd3e; N/A; N/A; N/A

cd3e; N/A; N/A; N/A

cd3e; N/A; N/A; N/A

Mnoclonal antibodies

Mouse (Mus musculus)

CD3 epsilon; N/A; CD3 epsilon; N/A; N/A

Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

For cells, cell lines and tissues in culture till half confluency.

Supplied as a liquid in PBS, pH 7.4, sodium azide. Conjugated to FITC.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse CD3e, Mr 25kD. Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of mouse strains tested.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.