CD3, epsilon (Early T Cell Marker) (Biotin)

525 EUR

500ug

GEN633346

N/A

5K3

cell

IgG,k

Hamster

Purified

Antibodies

Monoclonal

CD3, epsilon

Biotinylated

cd3e; N/A; N/A; N/A

cd3e; N/A; N/A; N/A

cd3e; N/A; N/A; N/A

Mnoclonal antibodies

Mouse (Mus musculus)

CD3 epsilon; N/A; CD3 epsilon; N/A; N/A

Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

For cells, cell lines and tissues in culture till half confluency.

Supplied as a liquid in PBS, pH 7.4, sodium azide. Conjugated to Biotin.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse CD3e, Mr 25kD. Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of mouse strains tested.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.