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- Anti-Mouse CD34, Biotin (Clone MEC14.7) (rat IgG2a)
Product short description
Price:
426 EUR
Size:
0.3 miligrams
Catalog no.:
GEN520493
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Gene name
CD34
Also known as
CD34
Form/Appearance
Biotin
Clone
MEC 14.7
Immunoglobulin isotype
Rat IgG2a
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Other gene names
CD34; CD34; N/A; N/A
Host organism
Rat (Rattus norvegicus)
Concentration
0.1 miligram per 1 mililiter
Latin name
Mus musculus,Rattus norvegicus
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Other names
CD34; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule; N/A
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Specificity and cross-reactivity
CD34 (mucosialin; sialomucin) (Mouse CD34); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
For all formats, store the antibody at +4 degrees Celsius. DO NOT FREEZE PE conjugates. For long term storage (Ascites, Purified, Biotin, FITC), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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