Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody

282 EUR

100ug

GEN525006

N/A

N/A

N/A

N/A

N/A

N/A

Flag

IgG1

FG4R

FLAG Tag

anticorps

Monoclonal

Antibodies

Mouse (Mus musculus)

Mnoclonal antibodies

Protein A affinity chromatography from mouse ascites fluid

Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody should be stored at -20 degrees Celsius. for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at Store the antibody at +4 degrees Celsius. (add 0.05% NaN3 if not included) for several days to weeks

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.