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- HAMSTER ANTI MOUSE CD28:FITC Antibody
Product short description
Price:
558 EUR
Size:
500ug
Catalog no.:
GEN210668
Product detailed description
Gene name synonims
N/A
Immunoglobulin isotype
IgG
Purification method
N/A
Also known as
CD28
Gene name
CD28
Clone
37.51.1
Host organism
Hamster
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Other gene names
Cd28; Cd28; N/A; N/A
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Concentration
IgG concentration 0.5 mg/ml
Form/Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Other names
T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; N/A
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD28 This item recognises murine CD28, an 80kDa cell surface glycoprotein that is expressed by most thymocyte subsets and by peripheral T-cells. Studies show that CD28 expression on thymocytes and splenic T-cells can be upregulated upon activation. Murine CD28 acts as a co-stimulatory signal and may play an important role in regulating the immune response of T-cells. Clone 37.51.1 has been reported to enhance cytokine production and T-cell proliferation in combination with TCR-mediated signals ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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