HAMSTER ANTI MOUSE CD28:Biotin Antibody

221 EUR

0.025 miligrams

GEN214962

N/A

IgG

N/A

CD28

CD28

37.51.1

Hamster

anticorps

Monoclonal

Antibodies

Biotinylated

Mus musculus

Cd28; Cd28; N/A; N/A

Mnoclonal antibodies

Flow Cytometry (FACS)

IgG concentration 0.1 miligram per 1 mililiter

Biotin (Purified IgG conjugated to Biotin - liquid)

T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; N/A

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.

CD28 This item recognises murine CD28, an 80kDa cell surface glycoprotein that is expressed by most thymocyte subsets and by peripheral T-cells. Studies show that CD28 expression on thymocytes and splenic T-cells can be upregulated upon activation. Murine CD28 acts as a co-stimulatory signal and may play an important role in regulating the immune response of T-cells. Clone 37.51.1 has been reported to enhance cytokine production and T-cell proliferation in combination with TCR-mediated signals ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.