FLAG (DYKDDDDK) Tag Antibody

332 EUR

100ug

GEN395220

N/A

N/A

N/A

N/A

N/A

Flag

FG4R

IgG2b

anticorps

Monoclonal

Antibodies

FLAG (DYKDDDDK) Tag

Mnoclonal antibodies

Mouse (Mus musculus)

Protein A/G Chromatography

10mM PBS, pH 7.2, with 0.05% Sodium Azide

Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS)

Product should be stored the antibody should be stored at -20 degrees Celsius.. Aliquot to avoid freeze/thaw cycles

FLAG tagged fusion proteins; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.