HLA Class 1 Antigen B27 (FITC) Antibody

824 EUR

100 Tests

GEN600254

N/A

IgG2a

3H2219

anticorps

Antibodies

Monoclonal

Anti-FITC Antibody

Mnoclonal antibodies

Mouse (Mus musculus)

Human (Homo sapiens)

HLA Class 1 Antigen B27

Flow Cytometry (FC/FACS)

HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB

HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB

HLA-B; HLA-B; AS; HLAB; SPDA1; HLAB

Highly Purified by Ion Exchange chromatography.

Antigens are peptides or recombinant or native dependent on the production method.

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

histocompatibility antigen alpha 1 domain; HLA class I histocompatibility antigen, B-7 alpha chain; major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain; major histocompatibility complex, class I, B; MHC class I antigen B*7

Recognizes HLA Class 1 Antigen B27. Affinity studies by Scatchard analysis showed that HLA-B27 antibody has a higher affinity for HLA-B27 (9.7 x 10e8M-1) than for HLA B7 (9.5 x 10e7 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.