Anti-FLAG Monoclonal Antibody

382 EUR

100ug

GEN430079

N/A

N/A

N/A

FLAG

Flag

anticorps

IgG1/kappa

Antibodies

Monoclonal

flaG; N/A; N/A; N/A

flaG; N/A; N/A; N/A

flaG; N/A; N/A; N/A

Mnoclonal antibodies

Mouse (Mus musculus)

Protein A & Ion exchange column

FlaG; N/A; Uncharacterized flagellar protein FlaG; N/A; N/A

Western Blot, Immunocytochemistry, Immunoprecipitation, Flow Cyctometry, protein purification, protein localization, etc

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.