Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti-FLAG)

199 EUR

0.01 mililiter

GEN435026

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Antibodies

Monoclonal

Mouse IgG2b

Agarose,Flag

Anti-DYKDDDDK-Tag

Mnoclonal antibodies

All; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

The product is supplied as a 50% slurry in storage buffer (1x PBS, pH 7.4, containing 0.1% NaN3). Store the product at Store the antibody at +4 degrees Celsius. and do not freeze.

Anti-DYKDDDDK-Tag Mouse mAb detects transfected proteins containing the DYKDDDDK epitope tag.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

The purest agarose was used in the production of Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti- ) by MBS Monoclonals.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.