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- HRP conjugated Anti-Flag
Product short description
Price:
514 EUR
Size:
100ug
Catalog no.:
GEN466017
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Form/Appearance
N/A
Also known as
Flag
Other names
FlaG; N/A
Clone
2F11-4H10
Category
Antibodies
Clonality
Monoclonal
Immunoglobulin isotype
IgG1, kappa
Tested applications:
Western Blot (WB)
Purification method
Protein G purified
Conjugation
Flag,HRP Conjugated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Concentration
1 miligram per 1 mililiter
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.
Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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