Anti-Mouse CD25, Biotin (Clone PC61.5.3) (rat IgG1)

260 EUR

100ug

GEN520077

N/A

N/A

N/A

CD25

CD25

PC61.5.3

Rat IgG1

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Rat (Rattus norvegicus)

Mus musculus,Rattus norvegicus

N/A; Pmp22; N/A; Cd25; Pmp-22; PMP-22; SAG

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

CD25; Peripheral myelin protein 22; N/A; N/A; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Biotin (Biotin conjugated purified Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ ml. (Purified from ascitic fluid via Protein G Chromatography))

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

CD25 (IL-2Ra, p55, Ly 43) (Mouse CD25 (IL-2 receptor, a chain)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.