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- Anti-Mouse CD27 Biotin (Clone LG.3A10) (Armenian Hamster IgG1)
Product short description
Price:
277 EUR
Size:
100ug
Catalog no.:
GEN520086
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Also known as
CD27
Gene name
CD27
Form/Appearance
Biotin
Clone
LG.3810
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Conjugation
Biotinylated
Host organism
Armenian Hamster
Subcategory
Mnoclonal antibodies
Immunoglobulin isotype
Armenian hamster IgG1
Concentration
0.1 miligram per 1 mililiter
Other gene names
CD27; CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2; TNFRSF7
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS) (stimulating)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
CD27 (Mouse CD27 Receptor); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Other names
CD27 antigen; CD27 antigen; CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; CD27L receptor; T-cell activation antigen CD27; T14; Tumor necrosis factor receptor superfamily member 7
Storage and shipping
For all formats, store the antibody at +4 degrees Celsius. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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