Anti-Rat CD4 Biotin (Clone W3/25) (mouse IgG1)

304 EUR

100ug

GEN520105

CD4

N/A

N/A

CD4

N/A

W3/25

Biotin

Antibodies

Monoclonal

Mouse IGg1

Biotinylated

Mouse (Mus musculus)

Mnoclonal antibodies

Mus musculus,Rattus norvegicus

CD4; CD4; CD4mut; CD4 receptor

Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Functional Assay (FA)

CD4; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4 molecule; N/A

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

CD4 (Rat CD4); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Store Ascites the antibody at -20 degrees Celsius. For all other formats, keep the antibody at +4 degrees Celsius. DO NOT FREEZE APC and PE conjugates. For long term storage (Purified, Biotin and FITC), aliquot and freeze at -20C. The unused portion of the antibody should be stored at -20 degrees Celsius in volumes suited for single use. Avoid freeze/defrost cycles.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.