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- MBS Monoclonals
- Anti-Bromodeoxyuridine (Brdu), FITC, (Clone: BU1/75 (ICR1)), (Rat IgG2a)
Product short description
Price:
                                                                                    376 EUR
                                                                            
                                Size:
                                        100ug
                                    
                                Catalog no.:
                                        GEN520464
                                    
                                Product detailed description
Gene name
                                        N/A
                                    
                                Gene name synonims
                                        N/A
                                    
                                Other gene names
                                        N/A
                                    
                                Concentration
                                        N/A
                                    
                                Other names
                                        N/A
                                    
                                Purification method
                                        Protein G
                                    
                                Immunoglobulin isotype
                                        Rat IgG2a
                                    
                                Category
                                        Antibodies
                                    
                                Clonality
                                        Monoclonal
                                    
                                Clone
                                        BU1/75 (ICR1)
                                    
                                Latin name
                                        Rattus norvegicus
                                    
                                Conjugation
                                        Anti-FITC Antibody
                                    
                                Subcategory
                                        Mnoclonal antibodies
                                    
                                Host organism
                                        Rat (Rattus norvegicus)
                                    
                                Also known as
                                        Bromodeoxyuridine (Brdu)
                                    
                                Tested applications:
                                        Immunocytochemistry (ICC)
                                    
                                Form/Appearance
                                        FITC (Purified Ig in PBS, 50% Glycerol, 0.1% Sodium Azide)
                                    
                                Species reactivity
                                        N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
                                    
                                Description
                                        This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
                                    
                                About
                                        Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
                                    
                                Specificity and cross-reactivity
                                        Bromodeoxyuridine (Brdu) (Reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. It also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not cross react with thymidine); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
                                    
                                Storage and shipping
                                        Store the antibody at +4 degrees Celsius. for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius. for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibodyStore the antibody at +4 degrees Celsius. for one month, or in small aliquots the antibody should be stored at -20 degrees Celsius. for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody
                                    
                                Properties
                                        This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
                                    
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