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- anti-mouse CD3-Biotin
Product short description
Price:
393 EUR
Size:
100ug
Catalog no.:
GEN666009
Product detailed description
Also known as
CD3
Gene name
CD3
Gene name synonims
N/A
Immunoglobulin isotype
IgG
Purification method
N/A
Host organism
Hamster
Clone
145-2C11
Concentration
1.0 mg/ml
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Conjugation
Biotinylated
Other gene names
N/A; cd3g; N/A; N/A
Subcategory
Mnoclonal antibodies
Other names
cd3; CD3gamma/delta; N/A; N/A; CD3gamma/delta
Tested applications:
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)
Form/Appearance
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4°C. Freezing/thawing cycles should be avoided in order to retain the full activity. Do not expose the vials to direct bright light. If stored under proper conditions, the antibody will retain its activity for at least one year.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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