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- MBS Monoclonals
- anti-human IgG-Biotin
Product short description
Price:
393 EUR
Size:
100ug
Catalog no.:
GEN666120
Product detailed description
Additional isotype
IgG
Gene name
IgG
Gene name synonims
N/A
Other gene names
N/A
Purification method
N/A
Also known as
IgG
Immunoglobulin isotype
IgG1
Clone
ICO-97
Other names
IgG; N/A
Concentration
1.0 mg/ml
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Tested applications:
Flow Cytometry (FC/FACS), ELISA (EIA)
Test
MBS Monoclonals supplies antibodies that are for research of human proteins.
Form/Appearance
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4°C. Freezing/thawing cycles should be avoided in order to retain the full activity. Do not expose the vials to direct bright light. If stored under proper conditions, the antibody will retain its activity for at least one year.
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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