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- MBS Monoclonals
- anti-mouse CD4-FITC
Product short description
Price:
393 EUR
Size:
120 Tests
Catalog no.:
GEN666176
Product detailed description
Also known as
CD4
Gene name
CD4
Gene name synonims
N/A
Purification method
N/A
Clone
GK1.5
Immunoglobulin isotype
IgG2bk
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Concentration
Volume: 0.2ml
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Other gene names
CD4; CD4; CD4mut; N/A
Host organism
Rat (Rattus norvegicus)
Tested applications:
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Form/Appearance
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Other names
CD4, partial; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4 molecule; CD4 protein
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4°C. Freezing/thawing cycles should be avoided in order to retain the full activity. Do not expose the vials to direct bright light. If stored under proper conditions, the antibody will retain its activity for at least one year.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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